NEET 2021 · Class 12

Biotechnology: Principles and Processes

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

  1. (A)Denaturation, Annealing, Extension
  2. (B)Denaturation, Extension, Annealing
  3. (C)Extension, Denaturation, Annealing
  4. (D)Annealing, Denaturation, Extension

The NCERT line that answers it

Verbatim line

**Figure 9.6** Polymerase chain reaction (PCR) : Each cycle has three steps: (i) Denaturation; (ii) Primer annealing; and (iii) Extension of primers

Chapter
Biotechnology: Principles and Processes
Section
9.3.3 Amplification of Gene of Interest using PCR
Page
11
Words matched
21 of 21

The answer appears as a continuous sentence in NCERT. This is that sentence.

How this line gives the answer

0, emb=1.0, combined=1.0). Verified: high — The span explicitly states 'Each cycle has three steps: (i) Denaturation; (ii) Primer annealing; and (iii) Extension of primers', which directly provides the correct sequence of steps in PCR and distinguishes it from the incorrect options.

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