NEET 2008 · Class 12

Biotechnology: Principles and Processes

44. The linking of antibiotic resistance gene with the plasmid vector became possible with

  1. (A)DNA polymerase
  2. (B)exonucleases
  3. (C)DNA ligase
  4. (D)endonucleases

The NCERT line that answers it

Verbatim line

After having cut the source DNA as well as the vector DNA with a specific restriction enzyme, the cut out 'gene of interest' from the source DNA and the cut vector with space are mixed and ligase is added.

Chapter
Biotechnology: Principles and Processes
Section
9.3.2 Cutting of DNA at Specific Locations
Page
11
Words matched
39 of 39

The answer appears as a continuous sentence in NCERT. This is that sentence.

How this line gives the answer

6, emb=0.9077, combined=0.7846). Verified: high — The span explicitly states that ligase is added to join the cut gene of interest with the vector DNA, which directly corresponds to DNA ligase's role in linking antibiotic resistance genes with plasmid vectors.

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