A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

(A)EcoRI
(B)Taq polymerase
(C)polymerase III
(D)ligase

The NCERT line that answers it

Verbatim line

When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of 'sticky-ends' and, these can be joined together (end-to-end) using DNA ligases (Figure 9.2).

Chapter: Biotechnology: Principles and Processes
Section: 9.2 Tools of Recombinant DNA Technology
Page: 6
Words matched: 29 of 29

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How this line gives the answer

0, emb=1.0, combined=1.0). Verified: high — The span explicitly states that DNA fragments with sticky ends produced by the same restriction enzyme can be joined together using DNA ligases, which directly answers the MCQ about joining foreign DNA and plasmid cut by the same restriction endonuclease.

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