Re-NEET 2026 · Question 162 · Class 12

Biotechnology: Principles and Processes

During PCR, primers bind to the DNA strands in the ______ step.

  1. (A)denaturation
  2. (B)extension
  3. (C)annealing
  4. (D)ligation

The NCERT line that answers it

Verbatim line

Annealing:** Primers (short DNA sequences) bind to the single strands at the boundaries of the region to be amplified.

Chapter
Biotechnology: Principles and Processes
Section
9.3.3 Amplification of Gene of Interest using PCR

The answer appears as a continuous sentence in NCERT. This is that sentence.

How this line gives the answer

0, emb=1.0, combined=1.0). Verified: high — The span explicitly states that primers bind to single strands during the annealing step, which directly answers the MCQ and distinguishes it from denaturation and extension.

We did this for 1,565 questions.

Every one traced to the exact sentence in NCERT that answers it, including 90% of the 2026 paper. Sit a mock on the real NEET 2027 computer interface and every mark you drop comes back with its line attached.

Take the free 5-minute checkMore from this chapter

Question text is reproduced from the published Re-NEET 2026 paper for study and commentary. NCERT text is quoted, not redistributed; textbooks remain at ncert.nic.in. Our mapping method, including its measured error rates, is published at /methodology.